3D platform for cell and tissue culture

Cultivating cells in conventional two-dimensional (2D) systems to mimic in vivo situations is a challenge. In this environment, not only cell-to-cell communication is lost, but also mechanical and biochemical properties. The goal of 3D (three-dimensional) cell culture is to provide an environment that accurately resembles the complex habitat that surrounding cells experience in their native tissues, allowing for in vitro growth, differentiation and functionalization.

3D platforms consisting of natural polymeric nanofibers are being increasingly used in areas such as tissue engineering, regenerative medicine, stem cell research and oncology, not only due to their structural similarity to the Extracellular Matrix (ECM), but also due to their high batch-to-batch consistency, their ability to shape biomaterials into different geometric shapes and the absence of animal derivatives.

Micrografia eletrônica - Colágeno Humano - Biocelltis Biotecnologia

Human collagen - Electron micrography

Nanofibras da Matriz 3D CellFate da Biocelltis Biotecnologia

Nanofibers of the 3D CellFate® matrix

Matrizes 3D CellFate da Biocelltis Biotecnologia são feitas para cultivar células humanas e animais em laboratório

Biocelltis’s 3D CellFate® matrices are developed for growing human and animal cells and tissues in lab.

The 3D CellFate® matrices developed by Biocelltis are biomaterials made up by natural polymeric nanofibers from 50 to 100 nm in diameter, similar to human collagen, biocompatible, sterile and ready-for-use, ideal for growing human and animal cells in research labs. Biocelltis offers 3D CellFate® matrices for a wide range of shapes and sizes of culture plates for routine use in laboratories, in addition to customized solutions.

Advantages of the 3D CellFate matrix®

  • Reproduce the physical structure of the extracellular matrix
  • Compatible with standard analytical methods
  • No need for specific handling equipment
  • Compatible with standard cell culture media and reagents
  • Product is sterile and ready for use
  • Xeno-free product
  • Room temperature storage
  • Shelf life of 24 months
As matrizes 3D CellFate da Biocelltis Biotecnologia reproduzem a estrutura física da matriz extracelular

Our results

Resultados apresentados pela matriz 3d CellFate da Biocelltis Biotecnologia

L929 fibroblasts cultured on the 3D CellFate® matrix. Image obtained using scanning electron microscopy (SEM).

Resultado apresentado pela matriz 3d CellFate da Biocelltis Biotecnologia

Human umbilical vein endothelial cells (HUVEC) cultured on the 3D CellFate® matrix and stained with the Live/Dead reagent. Green staining shows viable cells (calcein) and red staining shows dead cells.

Caso de sucesso da utilização da matriz 3d cellfate da biocelltis biotecnologia

Primary human fibroblasts cultured on the 3D CellFate® matrix with highlight to the cell nucleus stained in blue (DAPI) and cytoskeleton stained in red (phalloidin). Image obtained using confocal microscopy.